Hi Nagaraju,
This might not be the exact solution for your requirement, but may be you can try this out whether it works for you.
Why don't you create two page layouts for Opportunities and Assign these page layouts based on an existing standard field like say "Custom Status". When you select one specific Custom Status then you can assign a different page layout only for that specific Custom Status selection.
Go to the specific page, then click on Adapt --> Assign Page Layout. Select the Business Object as "Opportunity" and in the Instance Type you can select one of the existing field as a control object for changing the page layouts.
Using this option, you can have 2 different page layouts for the same business role, and the control object is the field selection that differentiates the page layout.
Regards,
Siraj